英文名字 Ras Activation Assay Biochem Kit
中文名称 Ras 活化检测生化试剂盒
供应商 Cytoskeleton
产品货号 BK008
产品报价 ¥11326/50assays
购买方式 Cytoskeleton中国区现货中心已经建立,大部分产品中国有现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-948
产品新闻
背景资料 待更新
产品描述 The Ras switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of Ras-like GTPases is of obvious biological significance and is a subject of intense investigation. The fact that Ras family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Ras protein activation. The assay uses the Ras-binding domain (RBD) of the Ras effector kinase Raf1. The Raf-RBD domain has been shown to bind specifically to the GTP-bound form of Ras proteins. The fact that the Raf-RBD has a high affinity for GTP-Ras and that its binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of Ras make it an ideal tool for affinity purification of GTP-Ras from cell lysates. The Raf-RBD is in the form of a GST fusion protein, which allows one to "pull-down" the Raf-RBD/GTP-Ras complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Ras activation in cells. The amount of activated Ras is determined by a western blot using a Ras specific antibody.
产品特点 待更新
保存建议 厂家推荐常温运输,收到产品后建议保存于4°C。
产品说明书
分享保存 分享按钮
其他 Cytoskeleton公司成立于1993年,专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰(PTM)、细胞骨架研究相关的系列试剂盒和产品,尤其以细胞骨架相关研究见长,既能满足于样品较少的科学研究,也可以用于小规模筛选研究和高通量大规模筛选研究。此外,公司还提供微管蛋白,肌动蛋白,小G蛋白,GAPs,GEFs等现有产品的药物筛选服务。

相关产品

购乐彩票 公司简介 新闻中心 产品中心 特色产品 技术专栏 在线订购 联系我们

版权所有 艾美捷科技购乐彩票

购乐彩票:武汉市洪山区光谷大道35号光谷总部国际二期时代1栋13楼 邮政编码:430074

电话:+86-027-59626688 全国免费技术支持: 400-6800-868 传真:+86-027-59716800

鄂ICP备10204150号

Abbkine Scientific   亚科因科技